Recombinant Vimentin anticorps

N° du produit ABIN7674794

Cet anticorps anti-Vimentin est un anticorps Souris Monoclonal détectant Vimentin dans WB, IHC, ICC, IHC (fro) et D. Adapté pour Souris.

Aperçu rapide pour Recombinant Vimentin anticorps (ABIN7674794)

Antigène: Vimentin (VIM)

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Vimentin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Detection (D)

Clone: RV204

Détail du produit anti-Vimentin anticorps

Fonction: Recombinant mouse anti vimentin

Specificité: Human and mouse vimentin

Attributs du produit: Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.

Purification: ProtA purified

Immunogène: This clone RV204 is a recombinant mouse anti vimentin monoclonal antibody produced in mammalian HEK293 cells. Clone RV204 is produced by cloning the antibody sequence of the mouse anti vimentin hybridoma RV202, which was derived by fusion of SP2/0-Ag14 mouse myeloma cells with spleen cells from a BALB/c mouse immunized with a cytoskeletal vimentin extract of calf lens.

Isotype: IgG1

Information d'application

Indications d'application: This clone RV204 is suitable for immunoblotting, immunocytochemistry, and immunohistochemistry on frozen and paraffin-embedd tissue sections. Optimal antibody dilution should be determined by titration, recommended range is 1:100 - 1:200 for immunohistochemistry with fluorochrome conjugated secondary antibodies or with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Reconstitution: Each vial contains 100 µL 1 mg/mL ProtA purified recombinant monoclonal antibody in PBS containing 0.09 % sodium azide. The purity of the antibody is >98 % as determined by SDS-Polyacrylamide gel electrophoresis. The endotoxin level is < 1EU/mg as determined by the LAL chromogenic endotoxin assay.

Stock: 4 °C,-20 °C

Stockage commentaire: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Détails sur Vimentin

Antigène: Vimentin (VIM)

Autre désignation: vimentin

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis